Response surface optimization of polysaccharide extraction from Galla Chinensis and determination of its antioxidant activity in vitro

Galla Chinensis was a traditional Chinese medicine used as an ingredient in many prescriptions for the treatment of oxidant damage-associated symptoms China. The current research on isolation polysaccharides from and their antioxidant activities still remain ongoing challenge. purpose this study is to develop technique determine properties vitro. An ultrasonic-assisted method extraction were firstly developed key parameters optimized response surface analysis crushing degree 100 mesh, ultrasonic time 20 min, materials/liquid ratio 1: 40. presented dose-dependent free radical scavenging effects DPPH, ABTS, total Iron ion reduction assays. Moreover, it also demonstrated effective protective effect human under H2O2-induced oxidative damage embryonic kidneyfibroblast 293 hepatoma 7721 cells by cell proliferation assay. determined Chinensis, thus providing new natural resource future investigation development polysaccharides-based drugs, health products or addictives.